Proteome analysis to assess physiological changes in Escherichia coli grown under glucose-limited fed-batch conditions.

Physiological and Morphological Changes of Recombinant E. coli During Over-Expression of Human Interferon-g in HCDC

The objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant E. coli. Batch and fed-batch culture techniques were employed to grow Escherichia coli BL21 for production of human gamma-interferon in pET expression system. Final cell densities in batch and fed-batch...

Fed-batch like cultivation in a micro-bioreactor: screening conditions relevant for Escherichia coli based production processes

OBJECTIVES Recombinant protein production processes in Escherichia coli are usually operated in fed-batch mode; therefore, the elaboration of a fed-batch cultivation protocol in microtiter plates that allows for screening under production like conditions is particularly appealing. RESULTS A highly reproducible fed-batch like microtiter plate cultivation protocol for E. coli in a micro-bioreac...

Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate

Growth rate related concentration changes of the starvation response regulators sigmaS and ppGpp in glucose-limited fed-batch and continuous cultures of Escherichia coli.

The entry of Escherichia coli into glucose starvation is connected to the induction of both the stringent response and the general stress response. The concentration of the corresponding response regulators ppGpp and sigmaS (RpoS, KatF) was investigated during glucose-limited growth at different growth rates and with different cultivation strategies, including fed-batch fermentation, chemostat,...

Systematic evaluation of characteristics of the membrane-based fed-batch shake flask

BACKGROUND The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and production scale must be as close as possible. To enable screening under fed-batch conditions, the membrane-based fed-batch shake flask was developed. It is a shake flask mounted...